This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques.
Basics of Extracellular Vesicles
Gain insight into a topic and learn the fundamentals.
4.8
(346 reviews)
Intermediate level
Some related experience required
Flexible schedule
Approx. 10 hours
Learn at your own pace
94%
Most learners liked this course
Skills you'll gain
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Assessments
5 assignments
Taught in English
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There are 5 modules in this course
In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles.
What's included
4 videos1 reading1 assignment
In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted.
What's included
6 videos1 assignment
In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids.
What's included
5 videos1 assignment
This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits.
What's included
6 videos1 assignment
This module highlights some of the different techniques used to characterize extracellular vesicles. Here the basic concepts for techniques such as electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits.
What's included
7 videos1 assignment
Instructor
Instructor ratings
4.6 (81 ratings)
Offered by
Recommended if you're interested in Basic Science
Erasmus University Rotterdam
University of Glasgow
Sungkyunkwan University
Universidad Nacional Autónoma de México
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Learner reviews
4.8
346 reviews
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Showing 3 of 346
MK
5
Reviewed on Aug 4, 2020
Awesome and unique course. Cleared my basics and introduced me to many new things. I especially loved knowing the different techniques used for EV research.
TG
5
Reviewed on Sep 28, 2016
I found this a very informative course on the recent developments and the basics of the EV field.
MR
5
Reviewed on Jan 10, 2019
Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.
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